Desmin anticorps

N° du produit ABIN4949599

L’anticorps Souris Monoclonal anti-Desmin a été validé pour WB, IF, IHC (p) et FACS. Il convient pour détecter Desmin dans des échantillons de Humain, Rat, Souris, Poulet et Hamster.

Aperçu rapide pour Desmin anticorps (ABIN4949599)

Antigène: Desmin (DES)

Reactivité: Humain, Rat, Souris, Poulet, Hamster

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Desmin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: DES-1711

Détail du produit anti-Desmin anticorps

Purification: Protein G affinity chromatography

Immunogène: Recombinant human Desmin was used as the immunogen for the Desmin antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the Desmin antibody should be determined by the researcher.\. WB: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Desmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur Desmin

Antigène: Desmin (DES)

Autre désignation: Desmin

Sujet: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.