Desmocollin 2+3 (Extracellular Domain) anticorps

N° du produit ABIN4949601

Cet anticorps Souris Monoclonal détecte spécifiquement dans IF, WB, FACS et IHC (p). Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour Desmocollin 2+3 (Extracellular Domain) anticorps (ABIN4949601)

Antigène: Desmocollin 2+3

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Immunofluorescence (IF), Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7G6

Détail du produit

Épitope: Extracellular Domain

Purification: Protein G affinity chromatography

Immunogène: A recombinant protein corresponding to the extracellular domain of human Desmocollin 2 was used as the immunogen for the Desmocollin 2/3 antibody.

Isotype: IgG1 kappa

Information d'application

Indications d'application: Optimal dilution of the Desmocollin 2/3 antibody should be determined by the researcher.\. WB: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the Desmocollin 2/3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détail du antigène

Antigène: Desmocollin 2+3

Autre désignation: Desmocollin 2/3

Sujet: Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. The desmosome is subdivided into two regions. The plaque region lies adjacent to the plasma, and is believed to contain molecules that attach the intermediate filament cytoskeleton to the desmosome. The core region is composed of transmembrane glycoproteins that are thought to mediate cell-cell adhesion. Desmogleins and desmocollins are the main desmosomal transmembrane proteins. These desmosomal glycoproteins belong to the members of the cadherin family of adhesion molecules. Three different isoforms of both desmogleins and desmocollins have been identified, named as desmoglein 1-3 and desmocollins. Desmosomal cadherins showed differentiation-specific expression in the human epidermis, although the functional significance of this differential expression is not fully understood. Desmocollin-1 can be found in the upper layers. The expression of Desmocollin-2 varies in the basal and suprabasal layers. And Desmocollin-3 is expressed more evenly throughout the suprabasal layers.