ENO2/NSE anticorps (AA 416-433)

N° du produit ABIN4949836

L’anticorps Souris Monoclonal anti-ENO2/NSE a été validé pour WB, IF, FACS et IHC (p). Il convient pour détecter ENO2/NSE dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour ENO2/NSE anticorps (AA 416-433) (ABIN4949836)

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ENO2/NSE est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ENO2-1375

Détail du produit anti-ENO2/NSE anticorps

Épitope: AA 416-433

Purification: Protein G affinity chromatography

Immunogène: Amino acids 416-433 of human Neuron Specific Enolase were used as the immunogen for this NSE antibody.

Isotype: IgG2b

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the NSE antibody to be titered up or down for optimal performance.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,IF: 1-2 μg/mL,IHC (FFPE): 0.1-0.2 μg/mL for 30 min at RT

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the NSE antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur ENO2/NSE

Antigène: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Autre désignation: NSE / Neuron Specific Enolase

Sujet: Recognizes a protein of about 50  kDa, which is identified as gamma-Enolase/Neuron Specific Enolase/Enolase 2. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue and gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.

ID gène: 2026