TDP2 anticorps

N° du produit ABIN4949896

L’anticorps Souris Monoclonal anti-TDP2 a été validé pour IF, FACS et IHC (p). Il convient pour détecter TDP2 dans des échantillons de Humain.

Aperçu rapide pour TDP2 anticorps (ABIN4949896)

Antigène: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TDP2 est non-conjugé

Application: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: TDP2-1258

Détail du produit anti-TDP2 anticorps

Purification: Protein G affinity chromatography

Immunogène: Human recombinant full length protein was used as the immunogen for this TDP2 antibody.

Isotype: IgG2b kappa

Information d'application

Indications d'application: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TDP2 antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Agent conservateur: Azide free

Stock: 4 °C,-20 °C

Stockage commentaire: Store the TDP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Détails sur TDP2

Antigène: TDP2 (tyrosyl-DNA phosphodiesterase 2 (TDP2))

Autre désignation: TDP2 / ETS1 associated protein II

Sujet: This mAb recognizes a protein of 41  kDa, which is identified as TDP2, or ETS1 associated protein II. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.