Estrogen Receptor alpha anticorps (Isoform 1)

N° du produit ABIN3029712

Cet anticorps anti-Estrogen Receptor alpha Polyclonal Lapin (ABIN3029712) détecte spécifiquement Estrogen Receptor alpha dans WB, ELISA et IF. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Estrogen Receptor alpha anticorps (Isoform 1) (ABIN3029712)

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Estrogen Receptor alpha est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Détail du produit anti-Estrogen Receptor alpha anticorps

Épitope: AA 452-480, Isoform 1

Fonction: ER Antibody

Réactivité croisée (Details): Expected species reactivity: Mouse, Rat, Pig

Purification: Antigen affinity

Immunogène: A portion of amino acids 452-480 from the human protein was used as the immunogen for this ER antibody (isoform 1).

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the ER antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the ER antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Détails sur Estrogen Receptor alpha

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: ER

Sujet: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements, the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Isoform 3 is involved in activation of NOS3 and endothelial nitric oxide production. Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full length receptor. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 can bind to ERE and inhibit isoform 1. [UniProt]

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly