ERK1/2 anticorps

N° du produit ABIN3031826

L’anticorps Souris Monoclonal anti-ERK1/2 a été validé pour WB et ELISA. Il convient pour détecter ERK1/2 dans des échantillons de Humain et Souris.

Aperçu rapide pour ERK1/2 anticorps (ABIN3031826)

Antigène: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ERK1/2 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 784CT7-6-3

Détail du produit anti-ERK1/2 anticorps

Purification: Purified

Immunogène: Purified His-tagged protein was used to produced this monoclonal ERK1/2 antibody.

Isotype: IgG2a

Information d'application

Indications d'application: Titration of the ERK1/2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the ERK1/2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur ERK1/2

Antigène: ERK1/2 (MAPK1/3) (Mitogen-Activated Protein Kinase 1/3 (MAPK1/3))

Autre désignation: ERK1/2

Sujet: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The the MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis.

UniProt: P27361