LC3B anticorps (pThr12)

N° du produit ABIN3031787

L’anticorps Lapin Polyclonal anti-LC3B a été validé pour ELISA et DB. Il convient pour détecter LC3B dans des échantillons de Humain.

Aperçu rapide pour LC3B anticorps (pThr12) (ABIN3031787)

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LC3B est non-conjugé

Application: ELISA, Dot Blot (DB)

Détail du produit anti-LC3B anticorps

Épitope: pThr12

Réactivité croisée (Details): Expected species reactivity: Bovine

Purification: Antigen affinity purified

Immunogène: This phospho-LC3B antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT12 of human LC3B.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the phospho-LC3B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Dot blot: 1:500

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the phospho-LC3B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur LC3B

Antigène: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Autre désignation: LC3B

Sujet: MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

UniProt: Q9GZQ8

Pathways: Autophagy