SMAD3 anticorps (AA 127-156)

N° du produit ABIN3032620

Cet anticorps Lapin Polyclonal détecte spécifiquement SMAD3 dans WB, ELISA, IHC et IF. Il présente une réactivité envers Humain.

Aperçu rapide pour SMAD3 anticorps (AA 127-156) (ABIN3032620)

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Détail du produit anti-SMAD3 anticorps

Épitope: AA 127-156

Réactivité croisée (Details): Expected species reactivity: Mouse, Rat, Pig, Chicken

Purification: Antigen affinity purified

Immunogène: A portion of amino acids 127-156 from the human protein was used as the immunogen for this SMAD3 antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the SMAD3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:10-1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the SMAD3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur SMAD3

Antigène: SMAD3 (SMAD, Mothers Against DPP Homolog 3 (SMAD3))

Autre désignation: SMAD3

Sujet: SMAD3 is a receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. [UniProt]

UniProt: P84022

Pathways: Cycle Cellulaire, Chromatin Binding, Cell-Cell Junction Organization, Positive Regulation of Endopeptidase Activity, Autophagy