TBP anticorps (AA 210-239)

N° du produit ABIN3029099

L’anticorps Lapin Polyclonal anti-TBP a été validé pour WB, ELISA, IHC, IF et FACS. Il convient pour détecter TBP dans des échantillons de Humain.

Aperçu rapide pour TBP anticorps (AA 210-239) (ABIN3029099)

Antigène: TBP (TATA Box Binding Protein (TBP))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TBP est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Détail du produit anti-TBP anticorps

Épitope: AA 210-239

Réactivité croisée (Details): Expected species reactivity: Bovine, Chicken, Mouse, Primate, Xenopus, Zebrafish

Purification: Purified

Immunogène: A portion of amino acids 210-239 from the human protein was used as the immunogen for this TBP antibody.

Isotype: Ig Fraction

Information d'application

Indications d'application: Titration of the TBP antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,Immunofluorescence: 1:10-1:50,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Aliquot the TBP antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Détails sur TBP

Antigène: TBP (TATA Box Binding Protein (TBP))

Autre désignation: TBP

Sujet: Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. A distinctive feature of TBP is a long string of glutamines in the N-terminal. This region of the protein modulates the DNA binding activity of the C terminus, and modulation of DNA binding affects the rate of transcription complex formation and initiation of transcription. Mutations that expand the number of CAG repeats encoding this polyglutamine tract, and thus increase the length of the polyglutamine string, are associated with spinocerebellar ataxia 17, a neurodegenerative disorder classified as a polyglutamine disease.

UniProt: P20226

Pathways: Signalisation WNT