Dystroglycan anticorps (Internal Region)

N° du produit ABIN498078

Cet anticorps anti-Dystroglycan Polyclonal Chèvre (ABIN498078) détecte spécifiquement Dystroglycan dans WB et EIA. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Boeuf (Vache) et Chien.

Aperçu rapide pour Dystroglycan anticorps (Internal Region) (ABIN498078)

Antigène: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Dystroglycan est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Dystroglycan anticorps

Épitope: Internal Region

Séquence: C-HVGKHEYFMH ATDK

Specificité: This antibody is expected to recognize both the precursor and the mature Alpha-Dystroglycan, but not the mature Beta-Dystroglycan.

Réactivité croisée (Details): Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide from the internal region of the protein sequence according to NP_004384.3. Genename: DAG1

Information d'application

Indications d'application: Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-0.6 μg/mL. Detects a band of Approx 100 kDa in Human Skeletal Muscle lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Dystroglycan

Antigène: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Autre désignation: Dystroglycan

Sujet: Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha Dystroglycan and a 43 kDa transmembrane protein, beta Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin.Synonyms: DAG1, Dystrophin-associated glycoprotein 1

ID gène: 1605

NCBI Accession: NP_004384

UniProt: Q14118

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus