Aconitase 1 anticorps

N° du produit ABIN498149

L’anticorps anti-Aconitase 1 Polyclonal Lapin est utilisé pour la détection de Aconitase 1 dans des échantillons de Humain, Souris et Rat. Il a été validé pour IHC (p) et IF.

Aperçu rapide pour Aconitase 1 anticorps (ABIN498149)

Antigène: Aconitase 1 (ACO1)

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Aconitase 1 est non-conjugé

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Détail du produit anti-Aconitase 1 anticorps

Specificité: This antibody detects endogenous levels of IRP-1 protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity Chromatography using epitope-specific immunogen.

Information d'application

Indications d'application: Western blot: 1/500-1/1000. Immunoflourescence: 1/50-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2 containing 0.05 % Sodium Azide as preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Aconitase 1

Antigène: Aconitase 1 (ACO1)

Autre désignation: ACO1 / IREB1

Sujet: The insulin receptor substrate-1 (IRS-1), a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor. The association of 14-3-3 with IRS-1 increases significantly upon treatment with okadaic acid, a potent serine/ threonine phosphatase inhibitor. Therefore, the association of 14-3-3 protein may play a role in the regulation of insulin sensitivity by interrupting the association between the insulin receptor and IRS-1.Synonyms: Aconitase, Citrate hydro-lyase, Cytoplasmic aconitate hydratase, Ferritin repressor protein, IRP1, Iron regulatory protein 1, Iron-responsive element-binding protein 1

Poids moléculaire: approx. 98 kDa

ID gène: 48

NCBI Accession: NP_002188

UniProt: P21399

Pathways: Transition Metal Ion Homeostasis