RAD52 anticorps

N° du produit ABIN498166

Cet anticorps Lapin Polyclonal détecte spécifiquement RAD52 dans WB, IF et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour RAD52 anticorps (ABIN498166)

Antigène: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RAD52 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-RAD52 anticorps

Specificité: This antibody detects endogenous levels of Rad52 protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur RAD52

Antigène: RAD52 (RAD52 Homolog (S. Cerevisiae) (RAD52))

Autre désignation: RAD52

Sujet: DNA double-strand breaks are generated by ionizing radiation and endogenously produced radicals, and they often are repaired through the RAD52 homologous recombination pathway. The RAD52 family includes RAD51, RAD52, RAD54, RAD54B and MRE11 genes. Rad51 and Rad52 proteins perform the key steps in homologous recombination (HR), including the search for DNA homology and strand exchange, through similar mechanisms. Mre11 functions in both non-homologous end joining, and meiotic HR, and it is essential in mitosis for chromosome maintenance. Rad54 belongs to the SWI2/SNF2 subfamily of ATPases, which includes the DNA helicases involved in replication, recombination, and repair, as it contains seven amino acid sequence motifs that are largely conserved. Rad54 ATPase activity is dependent on double-stranded (ds) DNA, and the ATPase activity of Rad54 is not absolutely required for its DNA repair function, suggesting that these activities occur at distinct regions of the molecule. RAD54B is significantly homologous to the RAD54 recombination gene. Expression of RAD54B is highest in testis and spleen, which are active in both meiotic and mitotic recombination.Synonyms: DNA repair protein RAD52 homolog

Poids moléculaire: approx. 46 kDa

ID gène: 5893

NCBI Accession: NP_602296

UniProt: P43351

Pathways: Réparation de l'ADN