TRF1 anticorps

N° du produit ABIN498269

L’anticorps anti-TRF1 Polyclonal Lapin est utilisé pour la détection de TRF1 dans des échantillons de Humain et Souris. Il a été validé pour WB et IHC (p).

Aperçu rapide pour TRF1 anticorps (ABIN498269)

Antigène: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-TRF1 anticorps

Specificité: This antibody detects endogenous levels of TRF1 protein.

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Pureté: > 95 % (by SDS-PAGE)

Information d'application

Indications d'application: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur TRF1

Antigène: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Autre désignation: TERF1

Sujet: Telomeric repeat binding factor 1 (TRF1, TERF1, PIN2, TRBF1) and telomeric repeat binding factor 2 (TRF2, TERF2, TRBF2) are present at telomeres throughout the cell cycle, where they regulate telomerase by acting in cis to limit the elongation of individual chromosome ends. Telomerase adds hexameric repeats of 5'-TTAGGG-3' to the ends of chromosomal DNA. This telomerase enzyme plays an influential role in cellular immortalization and cellular senescence. TRF1 negatively regulates telomere elongation, while TRF2 protects the chromosome ends by inhibiting end-to-end fusions. Downregulation of TRF expression in tumor cells may contribute to cell immortalization and malignant progression. TRF1 has an acidic N-terminus while TRF2 has a basic N-terminus. TRF2 localizes in the nucleolus at G0 and S and diffuses out of the nucleolus in G2 phase. During mitosis TRF2 disperses from the condensed chromosomes and returns to the nucleolus at cytokinesis.Synonyms: NIMA-interacting protein 2, PIN2, TRBF1, TRF, TRF1, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1, Telomeric repeat-binding factor 1

Poids moléculaire: approx. 50 kDa

ID gène: 7013

NCBI Accession: NP_003209

UniProt: P54274

Pathways: Cycle Cellulaire, Telomere Maintenance, Hormone Transport, Chromatin Binding, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity