DBF4 anticorps
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- Antigène Voir toutes DBF4 Anticorps
- DBF4 (DBF4 Homolog (DBF4))
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Reactivité
- Humain, Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp DBF4 est non-conjugé
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificité
- This antibody detects endogenous levels of Dbf4 protein. (region surrounding Ile18)
- Réactivité croisée (Details)
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Species reactivity (expected):Mouse.
Species reactivity (tested):Human. - Purification
- Affinity chromatography
- Pureté
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product DBF4 Anticorps primaire
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- Indications d'application
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Avoid repeated freezing and thawing.
- Stock
- 4 °C/-20 °C
- Stockage commentaire
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Antigène
- DBF4 (DBF4 Homolog (DBF4))
- Autre désignation
- DBF4 / ASK (DBF4 Produits)
- Synonymes
- anticorps ASK, anticorps CHIF, anticorps DBF4A, anticorps ZDBF1, anticorps RGD1305854, anticorps DBF4, anticorps LOC100229596, anticorps AA545217, anticorps Ask, anticorps dbf4a, anticorps xdbf4, anticorps ChDBF4, anticorps DBF4 zinc finger, anticorps DBF4 zinc finger L homeolog, anticorps DBF4, anticorps dbf4, anticorps Dbf4, anticorps dbf4.L
- Sujet
- The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.Synonyms: Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, Protein DBF4 homolog A, ZDBF1
- Poids moléculaire
- approx. 77 kDa
- ID gène
- 10926
- NCBI Accession
- NP_006707
- UniProt
- Q9UBU7
- Pathways
- Mitotic G1-G1/S Phases, DNA Replication
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