DBF4 anticorps

N° du produit ABIN498361

Cet anticorps Lapin Polyclonal détecte spécifiquement DBF4 dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain et Souris.

Aperçu rapide pour DBF4 anticorps (ABIN498361)

Antigène: DBF4 (DBF4 Homolog (DBF4))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DBF4 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-DBF4 anticorps

Specificité: This antibody detects endogenous levels of Dbf4 protein. (region surrounding Ile18)

Réactivité croisée (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur DBF4

Antigène: DBF4 (DBF4 Homolog (DBF4))

Autre désignation: DBF4 / ASK

Sujet: The Dbf4/Cdc7 protein kinase is essential for the activation of replication origins during S phase. Cdc7-Dbf4 efficiently phosphorylates several proteins that are required for the initiation of DNA replication, including five of the six minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase alpha-primase. This protein complex consists of the catalytic subunit Cdc7 associating with the regulatory and activating subunit Dbf4, and the kinase activity of the complex is regulated throughout the cell cycle mainly by fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the cell cycle, while the expression of Dbf4 is absent during G1 phase and accumulates during S and G2 phases. The anaphase-promoting complex rapidly degrades Dbf4 at the time of chromosome segregation, and the stability of Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of Dbf4 and the time of chromosome separation is important to ensuring that prereplicative complexes, which assemble after chromosome segregation, do not immediately refire.Synonyms: Activator of S phase kinase, Chiffon homolog A, DBF4-type zinc finger-containing protein 1, DBF4A, Protein DBF4 homolog A, ZDBF1

Poids moléculaire: approx. 77 kDa

ID gène: 10926

NCBI Accession: NP_006707

UniProt: Q9UBU7

Pathways: Mitotic G1-G1/S Phases, DNA Replication