BARD1 anticorps

N° du produit ABIN498379

Cet anticorps Lapin Polyclonal détecte spécifiquement BARD1 dans WB et IHC (p). Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour BARD1 anticorps (ABIN498379)

Antigène: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp BARD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Détail du produit anti-BARD1 anticorps

Specificité: This antibody detects endogenous levels of BARD1 protein. (region surrounding Arg8)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur BARD1

Antigène: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Autre désignation: BARD1

Sujet: Mutations within the BRCA1 gene, localized to chromosome 17q, are believed to account for approximately 45 % of families with increased incidence of both early-onset breast cancer and ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a RING domain near the N-terminus and appears to encode a tumor suppressor. BARD1 (BRCA1-associated RING domain protein 1) and BAP1 (BRCA1-associated protein 1) have both been shown to bind to the N-terminus of BRCA1 and are potential mediators of tumor suppression. BARD1 contains an N-terminal RING domain and three tandem ankyrin repeats. The C-terminus of BARD1 contains a region with sequence homology to BRCA1, termed the BRCT domain. BAP1 is an ubiquitin hydrolase and has been shown to enhance BRCA1-mediated cell growth suppression.Synonyms: BARD-1, BRCA1-associated RING domain protein 1

Poids moléculaire: approx. 85 kDa

ID gène: 580

NCBI Accession: NP_000456

UniProt: Q99728

Pathways: Réparation de l'ADN