LIG1 anticorps

N° du produit ABIN498444

Détail du produit anti-LIG1 anticorps

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of DNA Ligase I protein. (region surrounding Glu139)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur LIG1

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Autre désignation: DNA Ligase 1 (LIG1 Produits)

Synonymes: anticorps ATLIG1, anticorps DNA ligase 1, anticorps T6D22.23, anticorps ligI, anticorps AL033288, anticorps LigI, anticorps lig1, anticorps wu:fc54a11, anticorps DNA ligase 1, anticorps DNA ligase 1, putative, anticorps ligase I, DNA, ATP-dependent L homeolog, anticorps ligase I, DNA, ATP-dependent, anticorps LIG1, anticorps PB000674.02.0, anticorps PTRG_06243, anticorps PKH_140260, anticorps lig1.L, anticorps Lig1, anticorps lig-1, anticorps lig1

Sujet: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.Synonyms: DNA ligase 1, DNA ligase I, EC=6.5.1.1, LIG-1, LIG1, Polydeoxyribonucleotide synthase [ATP] 1

Poids moléculaire: 102,125 kDa

ID gène: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA