HLA-DOA anticorps

N° du produit ABIN498962

Détail du produit anti-HLA-DOA anticorps

Antigène: HLA-DOA (HLA Class II DO alpha (HLA-DOA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HLA-DOA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificité: This antibody detects endogenous levels of HLADOalpha protein. (region surrounding Val111)

Réactivité croisée (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Pureté: > 95 % by SDS-PAGE

Information d'application

Indications d'application: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur HLA-DOA

Antigène: HLA-DOA (HLA Class II DO alpha (HLA-DOA))

Autre désignation: HLA Class II DO alpha / HLA-DOA (HLA-DOA Produits)

Synonymes: anticorps HLA-DNA, anticorps HLA-DZA, anticorps HLADZ, anticorps major histocompatibility complex, class II, DO alpha, anticorps HLA-DOA

Sujet: Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigenpresenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH , preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.Synonyms: HLA class II histocompatibility antigen DO alpha chain, HLA-DNA, HLA-DZA, MHC DN-alpha, MHC DZ alpha, MHC class II antigen DOA

Poids moléculaire: approx. 34 kDa

ID gène: 3111

NCBI Accession: NP_002110

UniProt: P06340