Ephrin B2 anticorps (Internal Region)

N° du produit ABIN499146

Cet anticorps anti-Ephrin B2 est un anticorps Polyclonal produit chez le Chèvre, détectant Ephrin B2 dans WB et EIA. Convient pour Humain, Souris, Rat, Boeuf (Vache), Chien et Porc.

Aperçu rapide pour Ephrin B2 anticorps (Internal Region) (ABIN499146)

Antigène: Ephrin B2 (EFNB2)

Reactivité: Humain, Souris, Rat, Boeuf (Vache), Chien, Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp Ephrin B2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-Ephrin B2 anticorps

Épitope: Internal Region

Séquence: CPKVDSKTVG QYE

Specificité: Recognizes EFNB2

Réactivité croisée (Details): Species reactivity (expected):Canine, Bovine, Pig.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogène: Peptide with sequence from the internal region of the protein sequence according to NP_004084.1. Genename: EFNB2

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.1-0.3 μg/mL. This antibody detects a band of approx. 35 kDa band observed inHuman, Mouse and Rat Lung lysates.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur Ephrin B2

Antigène: Ephrin B2 (EFNB2)

Autre désignation: Ephrin-B2

Sujet: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP)and also interacts with PRKCABP and GRIP1. This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. Ephrin-B2 also binds EphA4, EphB1, EphB2, EphB3 and EphB4. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34+ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.Synonyms: EFNB2, EPH-related receptor tyrosine kinase ligand 5, EPLG5, HTK ligand, HTK-L, HTKL, LERK-5, LERK5

Poids moléculaire: 36.9kDa (NP_004084.1)

ID gène: 1948

NCBI Accession: NP_004084

UniProt: P52799

Pathways: Signalisation RTK, Regulation of Muscle Cell Differentiation