LIG1 anticorps (Internal Region)

N° du produit ABIN499175

L’anticorps anti-LIG1 Polyclonal Chèvre est utilisé pour la détection de LIG1 dans des échantillons de Humain et Rat. Il a été validé pour WB.

Aperçu rapide pour LIG1 anticorps (Internal Region) (ABIN499175)

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Reactivité: Humain, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp LIG1 est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-LIG1 anticorps

Épitope: Internal Region

Séquence: C-RVREDKQPEQ ATTS

Specificité: This antibody detects DNA ligase 1 (Internal).

Réactivité croisée (Details): Species reactivity (expected):RatSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogène: Peptide from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1

Information d'application

Indications d'application: Peptide ELISA: 1: 16000 (limit dilution). Western blot: 0.1-0.3 μg/mL. Approx 125 kDa band observed in nuclear lysates of cell lineHeLa (calculated MW of 105 kDa according to NP_000225.1). The observed molecularweight corresponds to earlier findings with different antibodies from other commercialsources.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Détails sur LIG1

Antigène: LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))

Autre désignation: DNA Ligase 1

Sujet: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I.Synonyms: DNA ligase 1, DNA ligase I, EC=6.5.1.1, LIG-1, LIG1, Polydeoxyribonucleotide synthase [ATP] 1

ID gène: 3978

NCBI Accession: NP_000225

UniProt: P18858

Pathways: Telomere Maintenance, Réparation de l'ADN, DNA Replication, Synthesis of DNA