ADAM10 anticorps (C-Term)

N° du produit ABIN499211

L’anticorps Lapin Polyclonal anti-ADAM10 a été validé pour WB, IF et EIA. Il convient pour détecter ADAM10 dans des échantillons de Humain.

Aperçu rapide pour ADAM10 anticorps (C-Term) (ABIN499211)

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADAM10 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-ADAM10 anticorps

Épitope: C-Term

Specificité: ADAM10 antibody was raised against a peptide corresponding to amino acids 732 to 748 of human ADAM10. This sequence is identical to those of bovine and rat origins and differs from that of mouse ADAM10 by one amino acid (2,4).

Purification: Ion exchange chromatography

Immunogène: Human ADAM10 (C-Terminus) Peptide

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: ADAM10 can be used for detection of ADAM10 at 1: 500 to 1: 2000 dilution. Anapproximately 85 kDa band can be detected, which may represent precursor. Immunocytochemistry: 2,0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur ADAM10

Antigène: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Autre désignation: CD156c / ADAM10

Sujet: Proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) contributes to a variety of inflammatory responses and programmed cell death. Notch receptor and its ligand participate in cell fate decisions during vertebrate development and are associated with several human disorders, including a T-cell lymphoma. TNF-alpha, notch and its ligand delta are all membrane-bond molecules, which are cleaved by proteases to release mature proteins or functional receptor. ADAM10, a metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease), was recently identified to cleave TNF-alpha, notch and its ligand delta (1-3). The genes encoding human, mouse, and bovine ADAM10 were recently cloned and designated ADAM 10, kuzbanian (KUZ), and MADM, respectively, (1,2,4). ADAM10 mRNA is expressed in a variety of human and bovine tissues (1,4).Synonyms: Disintegrin and metalloproteinase domain-containing protein 10, KUZ, Kuzbanian protein homolog, MADM, Mammalian disintegrin-metalloprotease

ID gène: 102

NCBI Accession: NP_001101

UniProt: O14672

Pathways: Signalisation Notch, EGFR Signaling Pathway