DFFA anticorps (C-Term)

N° du produit ABIN499742

L’anticorps Lapin Polyclonal anti-DFFA a été validé pour WB, IF et EIA. Il convient pour détecter DFFA dans des échantillons de Humain.

Aperçu rapide pour DFFA anticorps (C-Term) (ABIN499742)

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DFFA est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Détail du produit anti-DFFA anticorps

Épitope: C-Term

Specificité: DFF45 antibody was raised against a peptide corresponding to amino acids 313 to 331 of human DFF45.

Purification: DEAE

Immunogène: Human DFF45 (C-Terminus) Peptide

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: DFF45 antibody can be used for detection of DFF45 and one of the cleavedfragments of DFF45 at 1/1000 to 1/2000 dilution. A 45 kDa band can be detected innon-apoptotic cells. Immunocytochemistry. Immunoprecipitation: DFF45 antibody can be used for detection of DFF45 and one of thecleaved fragments of DFF45 at 1/1000 to 1/2000 dilution.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur DFFA

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Autre désignation: DFFA / ICAD

Sujet: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kDa DNA fragmentation factor (DFF45) was identified recently which was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD (2,3). Upon cleavage of DFF45/ICAD, a caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD

ID gène: 1676

NCBI Accession: NP_004392

UniProt: O00273

Pathways: Apoptose, Caspase Cascade in Apoptosis