Endonuclease G anticorps

N° du produit ABIN499796

L’anticorps Lapin Polyclonal anti-Endonuclease G a été validé pour WB, IHC (p) et EIA. Il convient pour détecter Endonuclease G dans des échantillons de Humain, Rat et Souris.

Aperçu rapide pour Endonuclease G anticorps (ABIN499796)

Antigène: Endonuclease G (ENDOG)

Reactivité: Humain, Rat, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Endonuclease G est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-Endonuclease G anticorps

Specificité: EndoG antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human EndoG.

Purification: Affinity chromatography purified via peptide column

Immunogène: Human EndoG Peptide

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western 1 to 2 μg/mL. HepG2 and 3T3 cell lysates can be used as positive control and a 35 kDa band can be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur Endonuclease G

Antigène: Endonuclease G (ENDOG)

Autre désignation: Endonuclease G

Sujet: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).Synonyms: ENDOG

ID gène: 2021

NCBI Accession: NP_004426

UniProt: Q14249

Pathways: Apoptose