DFFA anticorps (N-Term)

N° du produit ABIN499956

Cet anticorps anti-DFFA est un anticorps Lapin Polyclonal détectant DFFA dans WB, IHC (p) et EIA. Adapté pour Souris.

Aperçu rapide pour DFFA anticorps (N-Term) (ABIN499956)

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DFFA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Détail du produit anti-DFFA anticorps

Épitope: N-Term

Specificité: ICAD antibody was raised against a peptide corresponding to amino acids near the amino terminus of mouse ICAD .

Purification: Affinity chromatography purified via peptide column

Immunogène: Mouse ICAD (N-Terminus) Peptide

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western Blot: 0.5 - 1 μg/mL. A 45 kDa band can be detected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C.

Détails sur DFFA

Antigène: DFFA (DNA Fragmentation Factor, 45kDa, alpha Polypeptide (DFFA))

Autre désignation: DFFA / ICAD

Sujet: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which is cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF was identified as a DNase inhibitor designated ICAD, for inhibitor of caspase-activated DNase. Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is the hallmark of apoptotic cell death.Synonyms: DFF-45, DFF1, DFF45, DNA fragmentation factor 45 kDa subunit, DNA fragmentation factor subunit alpha, Inhibitor of CAD

ID gène: 13347

UniProt: O54786

Pathways: Apoptose, Caspase Cascade in Apoptosis