GAPDH anticorps

N° du produit ABIN5003194

L’anticorps Souris Monoclonal anti-GAPDH a été validé pour WB, IHC (p) et FACS. Il convient pour détecter GAPDH dans des échantillons de Humain, Souris, Rat et Porc. Il y a 1 publication disponible.

Aperçu rapide pour GAPDH anticorps (ABIN5003194)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain, Souris, Rat, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GAPDH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: 4F8

Détail du produit anti-GAPDH anticorps

Réactivité croisée: Humain, Souris, Porc, Rat

Purification: Purified by Protein G.

Immunogène: KLH conjugated synthetic peptide derived from human GAPDH

Isotype: IgG

Information d'application

Indications d'application: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption: 12 months

Références pour anticorps anti-GAPDH (ABIN5003194)

Cui, Hao, Li, Bao, Li, Gao, Gu: "Chronic Heat Stress Induces Immune Response, Oxidative Stress Response, and Apoptosis of Finishing Pig Liver: A Proteomic Approach." dans: International journal of molecular sciences, Vol. 17, Issue 5, (2017) (PubMed).

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH

Sujet:

Synonyms: Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, GAPD, CDABP0047, OK/SW-cl.12

Background: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

ID gène: 2597

UniProt: P04406