SKA1 anticorps (C-Term)

N° du produit ABIN500728

Cet anticorps Lapin Polyclonal détecte spécifiquement SKA1 dans WB et EIA. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour SKA1 anticorps (C-Term) (ABIN500728)

Antigène: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SKA1 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Détail du produit anti-SKA1 anticorps

Épitope: C-Term

Specificité: This antibody is specific to SKA1 and will not cross-react with SKA2 or SKA3.

Purification: Peptide Afiinity Chromatography

Immunogène: SKA1 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of Human SKA1.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Détails sur SKA1

Antigène: SKA1 (Spindle and Kinetochore Associated Complex Subunit 1 (SKA1))

Autre désignation: SKA1

Sujet: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing.Synonyms: C18orf24, Spindle and kinetochore-associated protein 1

ID gène: 220134

UniProt: Q96BD8

Pathways: M Phase