FAM33A anticorps (N-Term)

N° du produit ABIN500730

Détail du produit anti-FAM33A anticorps

Antigène: FAM33A (Spindle and Kinetochore-Associated Protein 2 (FAM33A))

Épitope: N-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp FAM33A est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificité: This antibody detects SKA2.

Réactivité croisée (Details): Species reactivity (tested):Human, mouse

Purification: Peptide affinity chromatography

Immunogène: SKA2 antibody was raised against a 15 amino acid peptide from near the amino terminus of human SKA2.

Isotype: IgG

Information d'application

Indications d'application: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Buffer: PBS containing 0.02 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: 4 °C/-20 °C

Stockage commentaire: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Détails sur FAM33A

Antigène: FAM33A (Spindle and Kinetochore-Associated Protein 2 (FAM33A))

Autre désignation: SKA2 (FAM33A Produits)

Synonymes: anticorps FAM33A, anticorps 1110001A07Rik, anticorps C78640, anticorps Fam33a, anticorps RGD1307084, anticorps fam33a, anticorps spindle and kinetochore associated complex subunit 2, anticorps Spindle and kinetochore-associated protein 2, anticorps SKA2, anticorps Ska2, anticorps ska2

Sujet: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Depletion of either SKA1 or SKA2 by siRNA results in the loss of both proteins from the KT, but does not impact overall KT structure. Cells depleted of the SKA complex undergo a prolonged checkpoint-dependent delay in a metaphase-like state, indicating the importance of the SKA complex in the maintenance of the metaphase plate and spindle checkpoint silencing. SKA2 has also been shown to interact with glucocorticoid receptors and to be involved in glucocorticoid signaling and cell proliferation.Synonyms: FAM33A, Spindle and kinetochore-associated protein 2

ID gène: 348235

NCBI Accession: NP_872426

UniProt: Q8WVK7

Pathways: M Phase