Ubiquitin anticorps (APC)

N° du produit ABIN5024464

L’anticorps Souris Monoclonal anti-Ubiquitin a été validé pour WB, IHC, ELISA, IF et ICC. Il convient pour détecter Ubiquitin dans des échantillons de Humain.

Aperçu rapide pour Ubiquitin anticorps (APC) (ABIN5024464)

Antigène: Ubiquitin (Ub)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Ubiquitin est conjugé à/à la APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: FK1

Détail du produit anti-Ubiquitin anticorps (APC)

Specificité: Detects polyubiquitinated proteins and ubiquitin chains. Does not detect monoubiquitinated proteins nor free ubiquitin.

Purification: Gel filtration

Immunogène: Ubiquitin conjugated lysozyme

Isotype: IgM

Information d'application

Indications d'application:

• WB (1:10000)
• optimal dilutions for assays should be determined by the user.

Commentaires:

A 1:5000 dilution of SMC-213 was sufficient for detection of ubiquitin conjugates in 2.5 μg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: 10 mM phosphate buffer, 0.15M NaCl pH 7.4, 0.1 % sodium azide, Storage buffer may change when conjugated

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Conjugated antibodies should be stored at 4°C

Détails sur Ubiquitin

Antigène: Ubiquitin (Ub)

Autre désignation: Ubiquitin

Sujet: Ubiquitin is a small protein found ubiquitously in all tissue types and acts as a post translational modification. It can bind to its substrate either as a single ubiquitin molecule or in a chain. It is involved in many regulatory processes, which include proteasomal degradation, signal transduction, DNA repair, endocytosis and autophagy.

ID gène: 7314

UniProt: P0CG47

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway