GRAP2 anticorps

N° du produit ABIN509570

Détail du produit anti-GRAP2 anticorps

Antigène: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp GRAP2 est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody UW40 recognizes GRAP2/GADS, a 41 kDa cytoplasmic adaptor protein that plays a pivotal role during the early events of signal transduction in T cells.

Réactivité croisée (Details): Human

Purification: Purified by protein-A affinity chromatography.

Pureté: > 95 % (by SDS-PAGE)

Immunogène: GST-fusion human GRAP2/GADS protein

Clone: UW40

Isotype: IgG2a

Information d'application

Indications d'application: Flow cytometry: Recommended dilution: 2-6 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Do not freeze.

Références pour anticorps anti-GRAP2 (ABIN509570)

Yankee, Yun, Draves, Ganesh, Bevan, Murali-Krishna, Clark: "The Gads (GrpL) adaptor protein regulates T cell homeostasis." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 173, Issue 3, pp. 1711-20, (2004) (PubMed).

Yankee, Draves, Ewings, Clark, Graves: "CD95/Fas induces cleavage of the GrpL/Gads adaptor and desensitization of antigen receptor signaling." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 12, pp. 6789-93, (2001) (PubMed).

Détails sur GRAP2

Antigène: GRAP2 (GRB2-Related Adaptor Protein 2 (GRAP2))

Autre désignation: GRAP2/GADS (GRAP2 Produits)

Synonymes: anticorps GADS, anticorps GRAP-2, anticorps GRB2L, anticorps GRBLG, anticorps GRID, anticorps GRPL, anticorps GrbX, anticorps Grf40, anticorps Mona, anticorps P38, anticorps Gads, anticorps GrpL, anticorps zgc:112091, anticorps GRAP2, anticorps grap2, anticorps zgc:65942, anticorps GRB2-related adaptor protein 2, anticorps GRB2-related adaptor protein 2a, anticorps GRB2-related adaptor protein 2b, anticorps GRAP2, anticorps Grap2, anticorps grap2a, anticorps grap2, anticorps grap2b

Sujet: GRB2 related adaptor protein 2,GRAP2/GADS (Grb2-related adaptor protein 2 / Grb2-related adaptor downstream of Shc) is a cytoplasmic adaptor protein containing N- and C-terminal SH3 domains flanking a central SH2 domain and a proline/glutamine-rich region. It is expressed predominantly in lymphoid tissue and hematopoietic cells, particularly in T cells. GRAP2/GADS plays a pivotal role during the early events of T cell signal transduction by recruiting the adaptor protein SLP-76 and its associated molecules, such as Vav, Nck, Itk, and ADAP, to the transmembrane adaptor protein LAT. GRAP2/GADS also binds several other signaling proteins, namely Gab2, HPK1 (hematopoietic progenitor kinase 1), and Cbl. Unlike similar adaptor protein Grb2, GRAP2/GADS shows higher selectivity when binding to the particular phosphorylated tyrosines of LAT adaptor.,GADS, GRID, GRAP-2, GrbX

ID gène: 9402

UniProt: O75791

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway