CD40 anticorps (N-Term)

N° du produit ABIN5518644

Cet anticorps Lapin Polyclonal détecte spécifiquement CD40 dans FACS et WB. Il présente une réactivité envers Humain et a été mentionné dans 2+ publications.

Aperçu rapide pour CD40 anticorps (N-Term) (ABIN5518644)

Antigène: CD40

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CD40 est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB)

Détail du produit anti-CD40 anticorps

Épitope: AA 20-37, N-Term

Fonction: Anti-CD40 Antibody Picoband®

Séquence: PEPPTACREK QYLINSQC

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-CD40 Antibody (ABIN5518644). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human CD40.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Clark, E. A. : CD40: a cytokine receptor in search of a ligand. Tissue Antigens 35: 33-36, 1990. 2. Harding, S. A., Sarma, J., Josephs, D. H., Cruden, N. L., Din, J. N., Twomey, P. J., Fox, K. A. A., Newby, D. E. : Upregulation of the CD40/CD40 ligand dyad and platelet-monocyte aggregation in cigarette smokers. Circulation 109: 1926-1929, 2004. 3. Stamenkovic, I., Clark, E. A., Seed, B. : A B-lymphocyte activation molecule related to the nerve growth factor receptor and induced by cytokines in carcinomas. EMBO J. 8: 1403-1410, 1989. 4. van Kooten, C., Banchereau, J. : CD40-CD40 ligand. J. Leuko. Biol. 67: 2-17, 2000. 5. Kato, K., Cantwell, M. J., Sharma, S., Kipps, T. J. : Gene transfer of CD40-ligand induces autologous immune recognition of chronic lymphocytic leukemia B cells. J. Clin. Invest. 101: 1133-1141, 1998.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-CD40 (ABIN5518644)

Meng, Li, Geng, Ohe, Yu, E, Ye, Yang, Kato, Zhang, Ishida, Ohta, Jin, Kakehata, Geng, Yamakawa: "Decreased expression of follicular dendritic cell-secreted protein correlates with increased immunoglobulin A production in the tonsils of individuals with immunoglobulin A nephropathy." dans: Translational research : the journal of laboratory and clinical medicine, Vol. 166, Issue 3, pp. 281-91, (2015) (PubMed).

Peng, Cheng, Zeng, Shi, Bao, Mao, Ji, Li, Guo, Liang, Wu: "The role of RANTES as a crucial downstream cytokine in calcineurin-dependent VSMC apoptosis stimulated by INFgamma and CD40L." dans: Cell biology international, Vol. 34, Issue 5, pp. 447-53, (2010) (PubMed).

Détails sur CD40

Antigène: CD40

Autre désignation: CD40

Sujet:

Synonyms: Tumor necrosis factor receptor superfamily member 5,B-cell surface antigen CD40,Bp50,CD40L receptor,CDw40,CD40,CD40,TNFRSF5,

Tissue Specificity: B-cells and in primary carcinomas.

Background: CD40 is a cell surface receptor that is expressed on the surface of all mature B cells and also expressed on monocytes, dendritic cells, and thymic epithelium. CD40 is a member of the tumor necrosis factor (TNF) receptor superfamily and is the receptor for CD40 ligand. CD40 ligand (CD40L, CD154, gp39, and TRAM) belongs to the TNF gene family and is expressed more widely than CD40 predominantly on activated CD4+ T cells. Activation of CD40 has also been shown to inhibit the growth of certain B cell lymphomas and to induce the death of transformed cells of mesenchymal or epithelial origin.

Sequence Similarities: Contains 4 TNFR-Cys repeats.

Poids moléculaire: 40 kDa

UniProt: P25942

Pathways: Signalisation NF-kappaB, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints