Cytokeratin 18 anticorps (AA 204-430)

N° du produit ABIN5518769

Cet anticorps anti-Cytokeratin 18 est un anticorps Lapin Polyclonal détectant Cytokeratin 18 dans WB, IHC, IF, FACS, ICC et IHC (fro). Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 6+ publications.

Aperçu rapide pour Cytokeratin 18 anticorps (AA 204-430) (ABIN5518769)

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Cytokeratin 18 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Détail du produit anti-Cytokeratin 18 anticorps

Épitope: AA 204-430

Fonction: Anti-Cytokeratin 18/KRT18 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-Cytokeratin 18/KRT18 Antibody Picoband® (ABIN5518769). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry, 0.5-1 μg/mL, Human
Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: KRT18 keratin 18". 2. Ku, N.-O., Wright, T. L., Terrault, N. A., Gish, R., Omary, M. B. Mutation of human keratin 18 in association with cryptogenic cirrhosis. J. Clin. Invest. 99: 19-23, 1997. 3. Ku, N.-O., Darling, J. M., Krams, S. M., Esquivel, C. O., Keeffe, E. B., Sibley, R. K., Lee, Y. M., Wright, T. L., Omary, M. B. Keratin 8 and 18 mutations are risk factors for developing liver disease of multiple etiologies. Proc. Nat. Acad. Sci. 100: 6063-6068, 2003.

Commentaires:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-Cytokeratin 18 (ABIN5518769)

Li, Li, Sun, Wu, Wang: "Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells." dans: Cell stress & chaperones, Vol. 21, Issue 3, pp. 439-52, (2017) (PubMed).

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." dans: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, (2017) (PubMed).

Wu, Guo, Hui, Ye, Xiang, Wan, Zou, Ma, Sun, Yang, Guo: "Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage." dans: Journal of veterinary science, Vol. 15, Issue 4, pp. 475-83, (2015) (PubMed).

Xu, Jin, Min, Li, Deng, Wu, Lin, Chen, Zhang, Li, Wang, Zhu, Wang, Chu, Shen, Li, Mao: "Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival." dans: Oncotarget, Vol. 6, Issue 4, pp. 2434-50, (2015) (PubMed).

Yao, Guo, Wang, Xu, Zhang, Li, Lai: "The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency." dans: Stem cells international, Vol. 2016, pp. 4148923, (2015) (PubMed).

Hu, Zhou, Shi, Zhu, Wang, Zhang, Hu, Qian, Xu: "Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues." dans: Oncology letters, Vol. 5, Issue 1, pp. 179-184, (2012) (PubMed).

Détails sur Cytokeratin 18

Antigène: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Autre désignation: KRT18

Sujet:

Synonyms: Keratin, type I cytoskeletal 18,Cell proliferation-inducing gene 46 protein,Cytokeratin-18,CK-18,Keratin-18,K18,KRT18,CYK18,PIG46,

Tissue Specificity: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma. .

Background: Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Poids moléculaire: 48 kDa

ID gène: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Cascade in Apoptosis