CTLA4 anticorps (AA 36-161)

N° du produit ABIN5518903

Détail du produit anti-CTLA4 anticorps

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Épitope: AA 36-161

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CTLA4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Fonction: Rabbit IgG polyclonal antibody for Cytotoxic T-lymphocyte protein 4(Ctla4) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.

Réactivité croisée (Details): No cross reactivity with other proteins.

Attributs du produit: Rabbit IgG polyclonal antibody for Cytotoxic T-lymphocyte protein 4(Ctla4) detection. Tested with WB, IHC-P, ELISA in Mouse,Rat.
Gene Name: cytotoxic T-lymphocyte-associated protein 4
Protein Name: Cytotoxic T-lymphocyte protein 4

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived mouse CTLA4 recombinant protein (Position: E36-D161). Mouse CTLA4 shares 67.5% amino acid (aa) sequence identity with human CTLA4.

Isotype: IgG

Information d'application

Indications d'application: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ELISA: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse

Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Commentaires:

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Détails sur CTLA4

Antigène: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Autre désignation: Ctla4 (CTLA4 Produits)

Synonymes: anticorps CTLA-4, anticorps CTLA4, anticorps CD152, anticorps Cd152, anticorps Ctla-4, anticorps Ly-56, anticorps CD, anticorps CELIAC3, anticorps GRD4, anticorps GSE, anticorps IDDM12, anticorps sCTLA4, anticorps cytotoxic T-lymphocyte associated protein 4, anticorps cytotoxic T-lymphocyte-associated protein 4, anticorps CTLA4, anticorps Ctla4

Sujet: CTLA-4(Cytotoxic T-Lymphocyte Antigen 4), also known as CD152, is a protein receptor that down regulates the immune system. It is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Blockade of CTLA4 expression in vitro augmented HIV-specific CD4-positive T-cell function.

Synonyms: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, Ctla4, Cd152

ID gène: 12477

UniProt: P09793

Pathways: Cancer Immune Checkpoints