MED8 anticorps (AA 1-195)

N° du produit ABIN5519031

Cet anticorps Lapin Polyclonal détecte spécifiquement MED8 dans WB, ELISA, IF, ICC, IHC et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour MED8 anticorps (AA 1-195) (ABIN5519031)

Antigène: MED8 (Mediator Complex Subunit 8 (MED8))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MED8 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-MED8 anticorps

Épitope: AA 1-195

Fonction: Anti-MED8 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-MED8 Antibody Picoband® (ABIN5519031). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human MED8 recombinant protein (Position: M1-R195).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC (Aug 2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase". ProcNatlAcadSci U S A. 99 (16): 10353-8. 2. Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology". Mol. Cell. 14 (5): 685-91.

Commentaires:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur MED8

Antigène: MED8 (Mediator Complex Subunit 8 (MED8))

Autre désignation: MED8

Sujet:

Synonyms: Mediator of RNA polymerase II transcription subunit 8, Activator-recruited cofactor 32 kDa component, ARC32, Mediator complex subunit 8, MED8,

Tissue Specificity: Ubiquitous.

Background: Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene. This gene encodes a protein component of the mediator complex, which aids in transcriptional activation through interaction with RNA polymerase II and gene-specific transcription factors. The encoded protein may also function in ubiquitin ligation and protein degradation.

Poids moléculaire: 29 kDa

ID gène: 112950

UniProt: Q96G25

Pathways: Regulation of Lipid Metabolism by PPARalpha