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MED15 anticorps (AA 1-285)

L’anticorps Lapin Polyclonal anti-MED15 a été validé pour WB, ELISA, IHC, IF, FACS et ICC. Il convient pour détecter MED15 dans des échantillons de Humain, Souris et Rat.
N° du produit ABIN5519035

Aperçu rapide pour MED15 anticorps (AA 1-285) (ABIN5519035)

Antigène

Voir toutes MED15 Anticorps
MED15 (Mediator Complex Subunit 15 (MED15))

Reactivité

  • 32
  • 8
  • 7
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
Humain, Souris, Rat

Hôte

  • 28
  • 5
  • 1
Lapin

Clonalité

  • 29
  • 5
Polyclonal

Conjugué

  • 24
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Cet anticorp MED15 est non-conjugé

Application

  • 21
  • 14
  • 7
  • 5
  • 5
  • 3
  • 1
  • 1
  • 1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)
  • Épitope

    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-285

    Fonction

    Anti-MED15 Antibody

    Réactivité croisée (Details)

    No cross-reactivity with other proteins.

    Attributs du produit

    Anti-MED15 Antibody (ABIN5519035). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

    Purification

    Immunogen affinity purified.

    Immunogène

    E. coli-derived human MED15 recombinant protein (Position: M1-A285).

    Isotype

    IgG
  • Indications d'application

    Western blot, 0.1-0.5 μg/mL, Human
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -

    Commentaires

    We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Antigène

    MED15 (Mediator Complex Subunit 15 (MED15))

    Autre désignation

    MED15

    Sujet

    Synonyms: Mediator of RNA polymerase II transcription subunit 15, Activator-recruited cofactor 105 kDa component, ARC105, CTG repeat protein 7a, Mediator complex subunit 15, Positive cofactor 2 glutamine/Q-rich-associated protein, PC2 glutamine/Q-rich-associated protein, TPA-inducible gene 1 protein, TIG-1, Trinucleotide repeat-containing gene 7 protein, MED15, ARC105, CTG7A, PCQAP, TIG1, TNRC7,

    Tissue Specificity: Expressed in all tissues examined, including heart, brain, lung, spleen, thymus, pancreas, blood leukocyte and placenta. However, the level of expression varied, with highest expression in the placenta and peripheral blood and lowest in the pancreas and kidney.

    Background: Mediator of RNA polymerase II transcription subunit 15, also known as Gal11, Spt13 in yeast and PCQAP, ARC105, or TIG-1 in humans is a protein encoded by the MED15 gene. The protein encoded by this gene is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. This gene contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.

    Poids moléculaire

    97 kDa

    ID gène

    51586

    Pathways

    Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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