MMP16 anticorps (AA 120-296)

N° du produit ABIN5519039

Cet anticorps anti-MMP16 est un anticorps Lapin Polyclonal détectant MMP16 dans WB, IHC, ELISA, FACS, ICC et IF. Adapté pour Humain, Souris et Rat. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour MMP16 anticorps (AA 120-296) (ABIN5519039)

Antigène: MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MMP16 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-MMP16 anticorps

Épitope: AA 120-296

Fonction: Anti-MMP16 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-MMP16 Antibody Picoband® (ABIN5519039). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E. coli-derived human MMP16 recombinant protein (Position: Y120-I296).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Sato, H., Tanaka, M., Takino, T., Inoue, M., Seiki, M. : Assignment of the human genes for membrane-type-1, -2, and -3 matrix metalloproteinases (MMP14, MMP15, and MMP16) to 14q12.2, 16q12.2-q21, and 8q21, respectively, by in situ hybridization. Genomics 39: 412-413, 1997. 2. Takino, T., Sato, H., Shinagawa, A., Seiki, M. : Identification of the second membrane-type matrix metalloproteinase (MT-MMP-2) gene from a human placenta cDNA library: MT-MMPs form a unique membrane-type subclass in the MMP family. J. Biol. Chem. 270: 23013-23020, 1995.

Commentaires:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Références pour anticorps anti-MMP16 (ABIN5519039)

Xue, Wu, Chen, Luo: "MT3-MMP down-regulation promotes tumorigenesis and correlates to poor prognosis in esophageal squamous cell carcinoma." dans: Cancer medicine, Vol. 5, Issue 9, pp. 2459-68, (2017) (PubMed).

Wang, Li, Wu, Wu, Li, Yang, Chen, Xie, Huang, Du, Zhou: "miR-132 can inhibit glioma cells invasion and migration by target MMP16 in vitro." dans: OncoTargets and therapy, Vol. 8, pp. 3211-8, (2015) (PubMed).

Détails sur MMP16

Antigène: MMP16 (Matrix Metallopeptidase 16 (Membrane-inserted) (MMP16))

Autre désignation: MMP16

Sujet:

Synonyms: Matrix metalloproteinase-16, MMP-16, 3.4.24.-, MMP-X2, Membrane-type matrix metalloproteinase 3, MT-MMP 3, MTMMP3, Membrane-type-3 matrix metalloproteinase, MT3-MMP, MT3MMP, MMP16, MMPX2,

Tissue Specificity: Expressed in heart, brain, placenta, ovary and small intestine. Isoform Short is found in the ovary.

Background: The matrix metalloproteinase 16 (MMP16) protein consists of 604 amino acids and has a characteristic MMP domain structure, which gene is mapped on human chromosome 8q21. Additionally, MMP16 has a C-terminal extension containing a potential transmembrane domain, similar to MMP14, MMP15, and MMP17. Furthermore, it is membrane-bound and is a member of the membrane-type MMPs that are a subclass in the MMP family since the other members lack a C-terminal transmembrane domain and are secreted as soluble forms. MMP16 is expressed as a 12-kb transcript in brain, placenta, heart, and some carcinoma cell lines, but is not detectably expressed in lung, kidney, liver, spleen, and muscle.

Poids moléculaire: 53 kDa

ID gène: 4325

UniProt: P51512