SIGLEC5 anticorps (C-Term)

N° du produit ABIN5534705

Détail du produit anti-SIGLEC5 anticorps

Antigène: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Épitope: AA 482-513, C-Term

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SIGLEC5 est non-conjugé

Application: Western Blotting (WB)

Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogène: This SIGLEC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 482-513 amino acids from the C-terminal region of human SIGLEC5.

Isotype: Ig Fraction

Information d'application

Indications d'application: For WB starting dilution is: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied in PBS with 0.09 % (W/V) sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Détails sur SIGLEC5

Antigène: SIGLEC5 (Sialic Acid Binding Ig-Like Lectin 5 (SIGLEC5))

Autre désignation: SIGLEC5 (SIGLEC5 Produits)

Synonymes: anticorps CD170, anticorps CD33L2, anticorps OB-BP2, anticorps OBBP2, anticorps SIGLEC-5, anticorps sialic acid binding Ig like lectin 5, anticorps sialic acid-binding Ig-like lectin 5, anticorps sialic acid binding Ig-like lectin 5, anticorps SIGLEC5, anticorps LOC100597560, anticorps Siglec5

Sujet: SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have one or more cytoplasmic immune receptor tyrosine-based inhibitory motifs (ITIM). SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6. Sequence analysis predicted that the 697-amino acid SIGLEC10 protein contains a signal peptide, an N-terminal V-set Ig-like domain and four C2-set Ig-like domains, five potential N-linked glycosylation sites, a transmembrane region, and a 126-residue cytoplasmic tail with 3 putative ITIMs. Northern blot analysis detected a major 3.0-kb SIGLEC10 transcript, with highest levels in spleen, lymph node, blood leukocytes, and appendix. Little or no expression was observed in pancreas, thyroid, and testis. Flow cytometric analysis demonstrated eosinophil-specific expression of SIGLEC10, but at a lower level than that of SIGLEC8. Expression was also detected on monocytes and a CD16-positive/CD56-negative natural killer-like lymphocyte population. After sialidase treatment, which is necessary for unmasking the sialic acid-binding site on SIGLECs interacting with cell surface sialic acids, cells expressing SIGLEC10 bound to red blood cells. Immunoprecipitation analysis indicated expression of a 100- to 120-kD monomeric protein, higher than the predicted molecular mass, suggesting that SIGLEC10 is glycosylated.

Poids moléculaire: 61 kDa

ID gène: 8778

UniProt: O15389