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C5AR1 anticorps (C-Term)

Verified C5AR1 Reactivité: Humain WB, FACS, ELISA, IHC, IF Hôte: Chèvre Polyclonal unconjugated
N° du produit ABIN5539670
  • Antigène Voir toutes C5AR1 Anticorps
    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
    Épitope
    • 16
    • 15
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivité
    • 116
    • 22
    • 19
    • 17
    • 14
    • 10
    • 5
    • 5
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Hôte
    • 80
    • 35
    • 21
    • 1
    Chèvre
    Clonalité
    • 78
    • 54
    • 2
    Polyclonal
    Conjugué
    • 62
    • 12
    • 11
    • 10
    • 7
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cet anticorp C5AR1 est non-conjugé
    Application
    • 72
    • 67
    • 39
    • 19
    • 18
    • 16
    • 13
    • 13
    • 10
    • 9
    • 8
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)
    Fonction
    C5AR1/CD88
    Séquence
    RESKSFTRST VDT
     Réactivité croisée
    Humain
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Classe de qualité
    Verified
    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

    Western Blot: Approx 38 kDa band observed in lysates of cell line HepG2 (calculated MW of 39.3 kDa according to NP_001727.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:64000.

    Commentaires

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/m

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Minimize freezing and thawing.
    Stock
    -20 °C
    Stockage commentaire
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Antigène
    C5AR1 (Complement Component 5a Receptor 1 (C5AR1))
    Autre désignation
    C5AR1 (C5AR1 Produits)
    Synonymes
    anticorps C5A, anticorps C5AR, anticorps C5R1, anticorps CD88, anticorps C5aR, anticorps C5r1, anticorps Cd88, anticorps D7Msu1, anticorps GPR77, anticorps complement C5a receptor 1, anticorps complement component 5a receptor 1, anticorps C5AR1, anticorps C5ar1, anticorps c5ar1
    Sujet
    C5AR1, C5A, C5AR, C5A-R, C5R1, CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin receptor, Complement component 5 receptor 1, Complement component 5a receptor 1, Anaphylatoxin receptor
    ID gène
    728, 113959
    NCBI Accession
    NP_001727
    Pathways
    Système du Complément
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