GAPDH anticorps (Internal Region) (Biotin)

N° du produit ABIN5539725

L’anticorps Chèvre Polyclonal anti-GAPDH a été validé pour WB, ELISA et IHC. Il convient pour détecter GAPDH dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour GAPDH anticorps (Internal Region) (Biotin) (ABIN5539725)

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivité: Humain, Souris, Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp GAPDH est conjugé à/à la Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Classe de qualité: Verified

Détail du produit anti-GAPDH anticorps (Biotin)

Épitope: Internal Region

Fonction: GAPDH (Internal), Biotinylated

Séquence: GVNHEKYDNS LK.

Specificité: This antibody is expected to recognize both reported isoforms (NP_002037.2, NP_001243728.1). Reported variants represent identical protein: NP_001276674.1, NP_002037.2, NP_001276675.1. GAPDH is constitutively expressed in almost all tissues at high levels

Réactivité croisée: Humain

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 36 kDa band observed in Human Liver lysates (calculated MW of 36.1 kDa according to NP_002037.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur GAPDH

Antigène: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Autre désignation: GAPDH

Sujet: GAPDH, glyceraldehyde-3-phosphate dehydrogenase, G3PD, GAPD, HEL-S-162eP, aging-associated gene 9 protein, epididymis secretory sperm binding protein Li 162eP, peptidyl-cysteine S-nitrosylase GAPDH

ID gène: 2597, 14433, 24383

NCBI Accession: NP_002037, NP_001243728