PRNP anticorps

N° du produit ABIN5540135

Cet anticorps Poulet Polyclonal détecte spécifiquement PRNP dans WB, ICC et IF. Il présente une réactivité envers Humain, Rat et Souris.

Aperçu rapide pour PRNP anticorps (ABIN5540135)

Antigène: PRNP (Prion Protein (PRNP))

Reactivité: Humain, Rat, Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Cet anticorp PRNP est non-conjugé

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

Détail du produit anti-PRNP anticorps

Purification: Purified

Immunogène: Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund's complete and incomplete adjuvants. After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks. Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached.Finally, affinity purified antibodies (50 ug/ml final concentration) were mixed with the IgY fraction (10 mg/ml final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% Sodium Azide.

Isotype: Ig Fraction

Information d'application

Indications d'application: Western Blot: 1/2500. Immunohistochemistry: 1/1000-1/5000. Quality Control: Antibodies were analyzed by Immunocytochemistry (1/1000 dilution) using cells transfected with the prion protein cDNA.

Restrictions: For Research Use only

Stockage

Buffer: Buffer System: 0.9 % (w/v) Sodium Phosphate (10 mM, pH 7.2) buffered isotonic saline, 50 % (v/v) Glycerol, with 0.02 % (w/v) Sodium Azide as an anti-microbial agent

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Upon receipt, store undiluted (in aliquots) at -20°C. This products is photosensitive and should be protected from light. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Détails sur PRNP

Antigène: PRNP (Prion Protein (PRNP))

Autre désignation: cd230,prp

Sujet: Prion protein is the protein responsible for human Creutzfeldt-Jakob Disease (CJD), and somatic mutations of the prion gene are responsible for Gerstmann-Straussler-Scheinker disease and Fatal Familial Insomnia. The bovine homolog of the prion protein is responsible for bovine Spongioform Encephalopathy (BSE), also known as Mad Cow Disease. In all cases, the prion protein has two folding states, with one state being the normal non-pathological form, and the other state inducing a spongioform encephalopathy.

UniProt: P23907

Pathways: Transition Metal Ion Homeostasis, Activated T Cell Proliferation