beta Actin anticorps (N-Term)

N° du produit ABIN5540759

Cet anticorps Souris Monoclonal détecte spécifiquement beta Actin dans WB, IF, IHC (p), ICC, FACS, IHC (fro) et EIA. Il présente une réactivité envers Humain, Souris, Rat, Poulet, Lapin, Porc et Poisson zèbre (Danio rerio).

Aperçu rapide pour beta Actin anticorps (N-Term) (ABIN5540759)

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivité: Humain, Souris, Rat, Poulet, Lapin, Porc, Poisson zèbre (Danio rerio)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp beta Actin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 4C2

Détail du produit anti-beta Actin anticorps

Épitope: N-Term

Séquence: Ac-DDDIAALVV-COOH

Specificité: This 4C2 Monoclonal antibody is highly specific for β-Cytoplasmic Actin. The antibody does not cross react with other Actin isoforms.

Purification: Purified

Immunogène: Peptide comprising the N-terminal nonapeptide of beta-Cytoplasmic Actin with an acetylated N-terminus coupled to KLH through the cysteine residue

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Flow cytometry. Western blot. Immunocytochemistry. Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin-embedded sections. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/100-1/500 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Détails sur beta Actin

Antigène: beta Actin (ACTB) (Actin, beta (ACTB))

Autre désignation: actin beta,actb

Sujet: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. β- and γ-cytoplasmic actin play crucial roles during various key cellular processes. Whereas β-actin is preferentially localized in stress fibers, circular bundles and at cell-cell contacts, suggesting a role in cell attachment and contraction, γ-actin displays a more versatile organization, according to cell activities. In moving cells, γ-actin is mainly organized as a meshwork in cortical and lamellipodial structures, suggesting a role in cell motility. β- and γ-actin depleted fibroblasts exhibit distinct changes in motility compared with their controls, suggesting a specific role for each isoform in cell locomotion.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction