Actin anticorps (N-Term)

N° du produit ABIN5540760

L’anticorps Souris Monoclonal anti-Actin a été validé pour WB, IHC (p), IHC (fro) et EIA. Il convient pour détecter Actin dans des échantillons de Humain, Rat, Lapin et Porc.

Aperçu rapide pour Actin anticorps (N-Term) (ABIN5540760)

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Reactivité: Humain, Rat, Lapin, Porc

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Actin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Clone: 3B3

Détail du produit anti-Actin anticorps

Épitope: N-Term

Séquence: Ac-DEDETTALVC -COOH

Specificité: This 3B3 Monoclonal antibody is highly specific for Alpha-Skeletal Actin, and does not cross react with other Actin isoforms.

Purification: Purified

Immunogène: Peptide comprising the N-terminal nonapeptide of alpha-skeletal Actin with an acetylated N-terminus coupled to keyhole limpet hemocyanin through the cysteine residue.

Isotype: IgG1

Information d'application

Indications d'application: ELISA. Flow Cytometry. Immunoblotting. Immunohistochemistry on Frozen Sections. Immunohistochemistry on Paraffin-Embedded Sections. Recommended Dilutions : 1/100-1/1000 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent and 1/1000-1/5000 for immunoblotting applications.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur Actin

Antigène: Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))

Autre désignation: alpha skeletal muscle actin,acta1

Sujet: Among the six actin isoforms described in mammals, two are found in virtually all cells (β- and γ-cytoplasmic), two are detected in smooth muscle cells (α- and γ-smooth muscle) and two are present in striated muscles, one predominantly in skeletal (α-skeletal) and one in cardiac (α-cardiac) muscle cells. These actin isoforms differ slightly in their N-terminus, but the sequence of each of these actins is highly conserved in higher vertebrates. Skeletal α-actin may also be expressed in the mammalian heart. Although during the late stage of fetal development α-cardiac actin becomes the main isoform in the myocardium, α-skeletal actin might still make up for almost half of its total striated muscle actin content.

Pathways: Caspase Cascade in Apoptosis, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development