Collagen IV anticorps

N° du produit ABIN5541082

Cet anticorps anti-Collagen IV est un anticorps Souris Monoclonal détectant Collagen IV dans WB, IHC (fro), IHC (p), FACS, IF et ICC. Adapté pour Humain, Porc et Boeuf (Vache).

Aperçu rapide pour Collagen IV anticorps (ABIN5541082)

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reactivité: Humain, Porc, Boeuf (Vache)

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Collagen IV est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Clone: 1043

Détail du produit anti-Collagen IV anticorps

Specificité: This antibody clone 1043 recognizes type IV collagen in the extracellular - basement membranes of normal and pathological tissues.

Réactivité croisée (Details): Does NOT react with Rat and Rabbit.

Purification: Purified

Immunogène: Purified Human type IV Collagen, isolated from Human placenta. Antibody derived by fusion of Sp 2/0 mouse myeloma cells with spleen cells from a BALB/c mouse.

Isotype: IgG1

Information d'application

Indications d'application: Western blot : 1/100-1/1000. Immunocytochemistry on fixed cells (methanol fixation). Flow cytometry. Immunohistochemistry on Frozen Sections using a PBS buffer containing 0.1 mM CaCl 2 and 0.1 mM MgCl. Immunohistochemistry on Paraffin Sections : Pretreatement with 1 mg/mL pepsin in 0.01N HCl for 30 minutes at room temperature is recommended. Recommended Dilutions : 1/50-1/100 for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Buffer System: PBS Preservatives: 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freeze-thaw cycles. Shelf life: One year from despatch.

Détails sur Collagen IV

Antigène: Collagen IV (COL4) (Collagen, Type IV (COL4))

Autre désignation: collagen type iv

Sujet: Type IV collagen is an integral part of most, if not all basement membranes. The extent of expression of the basement membrane component type IV collagen may be correlated with the degree of differentiation of basement membrane producing neoplasms. Neoplastic cells of invasive carcinomas often lack a continuous basement membrane. In diagnostic histopathology antibodies to type IV collagen may help to distinguish between non-invasive (benign or in situ) and invasive lesions. Furthermore the antiserum can be of help in the differential diagnosis of bullous lesions in dermatopathology.

UniProt: P02462