CD55 anticorps

N° du produit ABIN5541171

Détail du produit anti-CD55 anticorps

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Reactivité: Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CD55 est non-conjugé

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Functional Studies (Func)

Specificité: The monoclonal antibody RDIII-7 recognizes complement decay accelerating factor (DAF), also designated as CD55. RDIII-7 recognizes the common extracellular SCR region of CD55, detecting all isoforms of the protein.

Purification: Purified

Immunogène: NIH-3T3 cells expressing GPI-anchored Rat DAF

Clone: RDIII-7

Isotype: IgG1

Information d'application

Indications d'application: Flow Cytometry (Ref.2,3). Western blot: Non-reduced with a band size of 60-70 kDa. As positive control CHO-Rat DAF hyper-expressing cells were used (Ref.1-6). Functional Studies: RDIII-7 antibody completely blocked the protective effect of expressed Rat DAF (Ref.2,3,6). Immunofluorescence (Ref.4,5). Immunohistochemistry on Frozen Sections (Ref.3-5). The typical starting working dilution is 1/50.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Buffer System: PBS Stabilizers: 0.1 % BSA

Stock: 4 °C

Stockage commentaire: Store undiluted at 2-8°C. DO NOT FREEZE! Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur CD55

Antigène: CD55 (Complement Decay-Accelerating Factor (CD55))

Autre désignation: cd55,daf (CD55 Produits)

Synonymes: anticorps CR, anticorps CROM, anticorps DAF, anticorps TC, anticorps Daf, anticorps Daf-GPI, anticorps Daf1, anticorps GPI-DAF, anticorps CD55 molecule (Cromer blood group), anticorps CD55 molecule, decay accelerating factor for complement, anticorps CD55, anticorps Cd55

Sujet: Cells express on their surface several proteins which protect against complement attack, namely C receptor I (CR1), decay accelerating factor (DAF), membrane cofactor protein (MCP) and CD59. CR1, DAF and MCP regulate the activation pathways of complement by either accelerating decay of the C3 and C5 convertase (CR1, DAF), or acting as cofactors for the serine protease factor I, which cleaves and irreversibly inactivates C3b (CR1, MCP). Rat DAF (CD55) is a 60 kDa transmembrane protein that binds C3b and C4b to inhibit formation and half-life of the C3 convertases. It belongs to the receptors of complement activation (RCA) family. DAF is broadly distributed among cells in contact with plasma complment proteins, including both haematopoietic and nonhaematopoietic cells. Although DAF does not have an essential role in controlling hemolysis of erythrocytes, it has an important role in regulation of the deposition of C3 on nucleated cells. Together with other complement regulators DAF protects self cells from autologous complement-mediated injury. DAF cooperates with CD46 in circumventing autologous C3 deposition, while CD59 inhibits the pathway at the critical end-point.

UniProt: Q9QYJ9

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity