This antibody detects bovine trypsin. Cross reactivity from other sources is unknown. Immunoelectrophoresis give a single precipitin arc against anti-peroxidase, anti-rabbit serum as well as purified and partially purified trypsin [bovine pancreas].
Purification
Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
PRSS1
Reactivité: Humain
WB
Hôte: Lapin
Polyclonal
unconjugated
Indications d'application
ELISA : 1/14,000. Western blot: 1/1,000-1/5,000. Immunohistochemistry : Paraffin (FFPE) or Frozen sections 1/100 suggested to begin.
Restrictions
For Research Use only
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Stock
4 °C,-20 °C
Stockage commentaire
Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Date de péremption
12 months
Antigène
PRSS1
(Protease, serine, 1 (Trypsin 1) (PRSS1))
Autre désignation
trypsin-1,prss1
Sujet
Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen, but the active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin prefers lysine and arginine residues. One of the substrates for trypsin is chymotrypsinogen which is cleaved to produce active chymotrypsin. High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screen for and diagnose Cystic Fibrosis.