Topoisomerase II alpha anticorps (C-Term)

N° du produit ABIN5542013

Cet anticorps Souris Monoclonal détecte spécifiquement Topoisomerase II alpha dans WB, IF, IHC (p), FACS, IP et ICC. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Topoisomerase II alpha anticorps (C-Term) (ABIN5542013)

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Topoisomerase II alpha est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 1C5

Détail du produit anti-Topoisomerase II alpha anticorps

Épitope: C-Term

Specificité: This antibody detects 170 kDa of human Topo II alpha on Western blot with total cell lysate from human cell lines.

Réactivité croisée (Details): Does not rect with Mouse (WR19L, NIH/3T3), Rat (PC12, Rat-1) or Hamster (BHK) on Western blot.

Purification: Protein A agarose

Immunogène: Recombinant human Topoisomerase II alpha protein corresponding to C-termional 182 aa

Isotype: IgG2a

Information d'application

Indications d'application: Western blot: 0.1-0.5 μg/mL for chemiluminescence detection system. Immunoprecipitation: 1-10 μg/200-300 μg/mL of cell extract. Immunohoistochemistry on paraffin sections: Heat treatment is necessary Microwave oven 2 times for 10 minutes aech in 10 mM citrate buffer ( pH 6.5). Immunocytochemistry: 1-10 μg/mL. Flow cytometry: 0.1-1 μg/mL (final contentration).

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: PBS containing 50 % glycerol, pH 7.2. No preservative is contained.

Agent conservateur: Azide free

Stock: -20 °C

Stockage commentaire: Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.

Détails sur Topoisomerase II alpha

Antigène: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Autre désignation: top2a

Sujet: < div dir=ltr data-font-name=g_font_p0_4 data-canvas-width=519.3466821444035> Topoisomerase II alpha plays important roles in synthesis and transcription of DNA as well < span style=font-size: 12px> as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- and M-phases in transformed and developmentally regulated normal cells. Topoisomerase II alpha is also implicated in drug resistance of tumor cells.

UniProt: P11388

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases