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CRISPR-Cas9 anticorps

Name: CRISPR-Cas9 anticorps
SKU: ABIN5557542
Price: 502.31 EUR
Availability: InStock

Reactivité: Humain WB Hôte: Souris Monoclonal 1E8 unconjugated

Lane 1: 293T cells transfected with CRISPR9-Cas9, Lane 2: untransfected 293T cell lysate probed with CRISPR-Cas9 (1E8) Monoclonal Antibody, Unconjugated at 1:1000 overnight at 4˚C. (CRISPR-Cas9 anticorps)

1 image

N° du produit ABIN5557542

Fiche de données

Antigène

CRISPR-Cas9
Reactivité
20

2

1

1

1
Humain
Hôte
16

9
Souris
Anticorps secondaires appropriés
Clonalité
13

12
Monoclonal
Conjugué
17

2

1

1

1

1

1

1
Inconjugué
Application
23

10

8

7

5

4

3

2

1

1

1

1
Western Blotting (WB)

Réactivité croisée

Humain

Purification

Purified by Protein A.

Immunogène

Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (100-200aa)

Clone

1E8

Isotype

IgM
anti-CRISPR-Cas9 (N-Term) antibody
Reactivité: Streptococcus pyogenes WB, IF, IP, ICC Hôte: Souris Monoclonal 7A9-3A3 unconjugated

anti-CRISPR-Cas9 (AA 1-462) antibody
Reactivité: Staphylococcus aureus WB Hôte: Souris Monoclonal unconjugated Recombinant Antibody

anti-CRISPR-Cas9 (C-Term) antibody
Reactivité: Streptococcus pyogenes WB, ELISA, FM Hôte: Lapin Polyclonal unconjugated

anti-CRISPR-Cas9 (C-Term) antibody (HRP)
Reactivité: Streptococcus pyogenes WB, ELISA, FM Hôte: Lapin Polyclonal HRP

anti-CRISPR-Cas9 (C-Term) antibody (Biotin)
Reactivité: Streptococcus pyogenes WB, ELISA, FM Hôte: Lapin Polyclonal Biotin

anti-CRISPR-Cas9 antibody
Reactivité: Staphylococcus aureus WB, IF Hôte: Lapin Polyclonal unconjugated

anti-CRISPR-Cas9 antibody
Reactivité: Streptococcus pyogenes WB, IF, IP, ChIP Hôte: Souris Monoclonal 8C1-F10 unconjugated

anti-CRISPR-Cas9 (C-Term) antibody (DyLight 800)
Reactivité: Streptococcus pyogenes WB, ELISA, FM Hôte: Lapin Polyclonal DyLight 800

anti-CRISPR-Cas9 (C-Term) antibody (DyLight 549)
Reactivité: Streptococcus pyogenes WB, ELISA, FM Hôte: Lapin Polyclonal DyLight 549

anti-CRISPR-Cas9 (C-Term) antibody (DyLight 680)
Reactivité: Streptococcus pyogenes WB, ELISA, FM Hôte: Lapin Polyclonal DyLight 680

Indications d'application

WB 1:500-2000

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

Aqueous buffered solution containing 1xTBS ( pH 7.4), 1 % BSA, 40 %Glycerol and 0.05 % Sodium Azide.

Stock

4 °C,-20 °C

Stockage commentaire

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Date de péremption

12 months
Antigène

CRISPR-Cas9

Sujet

Synonyms: CRISPR, Cas9, CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, SpCas9, csn1
Background: CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

ID gène

901176

UniProt

Q99ZW2