CRISPR-Cas9 (N-Term) anticorps

N° du produit ABIN5563965

L’anticorps anti-CRISPR-Cas9 Monoclonal Souris est utilisé pour la détection de CRISPR-Cas9 dans des échantillons de Streptococcus pyogenes. Il a été validé pour WB, IF, IP et ChIP.

Aperçu rapide pour CRISPR-Cas9 (N-Term) anticorps (ABIN5563965)

Antigène: CRISPR-Cas9

Reactivité: Streptococcus pyogenes

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Chromatin Immunoprecipitation (ChIP)

Clone: 8C1-F10

Détail du produit

Épitope: N-Term

Fonction: Cas9 antibody (mAb) (Clone 8C1-F10)

Specificité: Mouse

Purification: Protein A Chromatography

Immunogène: This antibody was raised against a recombinant protein within the N-terminal region of Streptococcus pyogene Cas9. This antibody should recognize Cas9 and dCas9 based on the antigen design.

Isotype: IgG2b

Information d'application

Indications d'application: ChIP ICC/IF IP WB Validation was done using crude hybridoma supernatant. Individual optimization required. For Cas9, we also offer AbFlex Cas9 Recombinant Antibody (rAb). For details, see Catalog No. 91123.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Détail du antigène

Antigène: CRISPR-Cas9

Autre désignation: Cas9

Sujet: Cas9 is a nuclease from Streptococcus pyogenes that can be targeted to particular DNA sequences through a guide RNA that results in double-stranded breaks in DNA. Cas9 is part of the CRISPR/Cas9 gene-editing system that can create a DNA break at a specific location with the genome. CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) Probable. In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Poids moléculaire: 160 kDa

NCBI Accession: WP_014407541