Recombinant Rpb1 CTD anticorps (pSer2)

N° du produit ABIN5563955

Détail du produit anti-Rpb1 CTD anticorps

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Type d'anticorp: Recombinant Antibody

Épitope: pSer2

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Rpb1 CTD est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP), ChIP DNA-Sequencing (ChIP-seq), ELISA

Marque: AbFlex®

Attributs du produit: AbFlex™ RNA pol II CTD phospho Ser2 antibody was expressed as full-length IgG with mouse immunoglobulin heavy and light chains (IgG2a isotype) in mammalian 293 cells. RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at two sites within the heptapeptide repeat, at serine 2, serine 5 and serine 7. RNA pol II phosphorylated at serine 2 is enriched over the gene body and is associated with transcriptional elongation.

Purification: Ni-NTA

Isotype: IgG2a

Information d'application

Indications d'application: ChIP-Seq: 4 μg each ICC/IF: 1 - 2 μg / mL WB: 0.5 - 2 μg/mL Bead-based ELISA: 0.1 - 5 μg/mL

Commentaires:

AbFlex® Recombinant Antibodies defined antibodies for highly specific, reproducible performance.
AbFlex® antibodies are recombinant antibodies (rAbs) that have been generated using defined DNA sequences to produce highly specific, reproducible antibodies. The unique advantages of the AbFlex® antibody are its flexible labeling and purification options. Each AbFlex® antibody contains a Sortase recognition motif (LPXTG) to covalently add fluorophores, enzymatic substrates (HRP, AP...etc), peptides, DNA, drugs or other labels to the antibody in a directed and reproducible manner using our Sortag-IT Labeling Kits. Every antibody also contains a 6xHis tag, which can be used with nickel-based purification systems, and an avidin tag sequence for enzymatic biotin conjugation using the biotin ligase, BirA.

AbFlex® antibodies are specifically labeled at the end of the constant region of the heavy chain to avoid interference with antigen recognition and functionality. This is important as it ensures the labeling process maintains the integrity of the antibody so signal is not diminished as a result of non-functional antibodies. In contrast, commonly used chemical labeling methods add labels to the antibody in a random fashion. The randomness of this process has a high potential to block the antigen-binding site and render the antibody ineffective. Chemical labeling can also deposit labels on the Fc region of the antibody which has the potential to obstruct interactions with protein A.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in 140 mM Hepes, pH 7.5, 70 mM NaCl, 35 mM NaOAc, 0.035 % sodium azide, 30 % glycerol

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Détails sur Rpb1 CTD

Antigène: Rpb1 CTD (RNA Polymerase II CTD)

Autre désignation: RNA pol II CTD (Rpb1 CTD Produits)

Synonymes: anticorps 5, anticorps 8WG16, anticorps CG1554, anticorps CTD, anticorps Dmel\\CG1554, anticorps H5, anticorps II, anticorps L5, anticorps POL, anticorps Pol II, anticorps Pol II CTD, anticorps Pol II Ser5P, anticorps Pol II0[ser2], anticorps Pol II0[ser5], anticorps Pol II[ser2], anticorps Pol IIa, anticorps Pol IIo, anticorps Pol IIo[Ser2], anticorps Pol IIo[ser2], anticorps Pol IIo[ser5], anticorps Pol-IIa, anticorps PolII, anticorps PolIIo, anticorps RNA Pol II, anticorps RNA Pol II CTD, anticorps RNA PolI 215, anticorps RNA PolII, anticorps RNA pol II, anticorps RNA pol IIo, anticorps RNA polII, anticorps RNAP, anticorps RNAP II, anticorps RNAP II LS, anticorps RNAPII, anticorps RNAPII0, anticorps RNApol2, anticorps RNApolII, anticorps RPB1, anticorps RPII215, anticorps RpII, anticorps Rpb1, anticorps Rpll215, anticorps Ubl, anticorps dRPB1, anticorps dRpb1, anticorps l(1)10Ca, anticorps l(1)DC912, anticorps l(1)DF912, anticorps l(1)G0040, anticorps l(1)L5, anticorps pol II, anticorps polII, anticorps RNA polymerase II 215kD subunit, anticorps RpII215

Poids moléculaire: 240 kDa