TET3 anticorps (AA 976-1521)

N° du produit ABIN5563988

Cet anticorps Rat Monoclonal détecte spécifiquement TET3 dans WB, IF, IP et ICC. Il présente une réactivité avec des échantillons de Souris.Il a été cité dans 1 publication.

Aperçu rapide pour TET3 anticorps (AA 976-1521) (ABIN5563988)

Antigène: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Reactivité: Souris

Hôte: Rat

Clonalité: Monoclonal

Conjugué: Cet anticorp TET3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 23B9

Détail du produit anti-TET3 anticorps

Épitope: AA 976-1521

Fonction: Tet3 antibody (mAb) (Clone 23B9)

Specificité: Mouse

Purification: Protein G Chromatography

Immunogène: This antibody was raised against a recombinant protein corresponding amino acids 976-1521 of mouse Tet3.

Isotype: IgG2a

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Stock: -20 °C

Stockage commentaire: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Date de péremption: 24 months

Références pour anticorps anti-TET3 (ABIN5563988)

Bauer, Göbel, Nagaraj, Colantuoni, Wang, Müller, Kremmer, Rottach, Leonhardt: "Phosphorylation of TET proteins is regulated via O-GlcNAcylation by the O-linked N-acetylglucosamine transferase (OGT)." dans: The Journal of biological chemistry, Vol. 290, Issue 8, pp. 4801-12, (2015) (PubMed).

Détails sur TET3

Antigène: TET3 (Tet Methylcytosine Dioxygenase 3 (TET3))

Autre désignation: Tet3

Sujet: Tet3 (Tet Methylcytosine Dioxygenase 3) is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in epigenetic chromatin reprogramming in the zygote following fertilization. Also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Conversion of 5mC into 5hmC, 5fC and 5caC probably constitutes the first step in cytosine demethylation. In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process. Participates in DNA demethylation in the paternal pronucleus by mediating conversion of 5mC into 5hmC, 5fC and 5caC. Does not mediate DNA demethylation of maternal pronucleus because of the presence of DPPA3/PGC7 on maternal chromatin that prevents TET3-binding to chromatin (By similarity). In addition to its role in DNA demethylation, also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT.

Poids moléculaire: 180 kDa

NCBI Accession: NP_898961

Pathways: L'effet Warburg