ADA anticorps (HRP)

N° du produit ABIN5596683

N° du produit (Fournisseur): 200-403-140-0100

Cet anticorps Lapin Polyclonal détecte spécifiquement ADA dans WB, ELISA et IP. Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour ADA anticorps (HRP) (ABIN5596683)

Antigène: ADA (Adenosine Deaminase (ADA))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp ADA est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Détail du produit anti-ADA anticorps (HRP)

N° du produit (Fournisseur): 200-403-140-0100

Fournisseur: Rockland

Fonction: Adenosine Deaminase Antibody Peroxidase Conjugated

Attributs du produit: Synonyms: rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase

Purification: Anti-Adenosine deaminase Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 μg of Adenosine Deaminase [Calf Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.

Immunogène:

Immunogen: Adenosine Deaminase [Calf Spleen] ADA

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Anti-Adenosine deaminase has been assayed against 1.0 μg of Adenosine Deaminase [Calf Spleen] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur ADA

Antigène: ADA (Adenosine Deaminase (ADA))

Autre désignation: ADA

Sujet: Background: Adenosine deaminase (adenosine aminhydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. Inosine can then be deribosylated by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine.

ID gène: 280712

NCBI Accession: NP_776312

UniProt: P56658

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process