PCK1 anticorps (HRP)
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- Antigène Voir toutes PCK1 Anticorps
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
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Reactivité
- Zea mays
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Cet anticorp PCK1 est conjugé à/à la HRP
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Réactivité croisée (Details)
- Cross reactivity against Phospho-enol-Pyruvate Carboxylase from other tissues and species may occur but have not been specifically determined.
- Purification
- Phospho Enol Pyruvate Carboxylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Phospho-enol-Pyruvate Carboxylase [Maize].
- Immunogène
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Immunogen: Phospho-enol-pyruvate Carboxylase [Maize Leaves]
Immunogen Type: Native Protein
- Isotype
- IgG
- Top Product
- Discover our top product PCK1 Anticorps primaire
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- Indications d'application
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Immunohistochemistry Dilution: User Optimized
Application Note: Anti-Phosphoenolpyruvate Carboxylase has been assayed against 1.0 μg of Phospho-enol-Pyruvate Carboxylase [Maize] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:6,000 of the reconstitution concentration is suggested for this product.
Western Blot Dilution: 1:500 - 1:2,000
ELISA Dilution: 1:5,000 - 1:20,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
- Agent conservateur
- Gentamicin sulfate
- Précaution d'utilisation
- This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
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- Antigène
- PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))
- Autre désignation
- Phospho Enol Pyruvate Carboxylase (PCK1 Produits)
- Synonymes
- anticorps PEPCK-C, anticorps PEPCK1, anticorps PEPCKC, anticorps PEPCK, anticorps PEPCK-M, anticorps PEPCK2, anticorps 1810010O14Rik, anticorps 9130022B02Rik, anticorps cb856, anticorps cb924, anticorps fj93h11, anticorps zgc:63869, anticorps wu:fc51c05, anticorps wu:fj93h11, anticorps pepck-c, anticorps pepck1, anticorps pepckc, anticorps PCK1, anticorps PPCK1, anticorps AI265463, anticorps Pck-1, anticorps GTP, anticorps PCK, anticorps Pepck, anticorps RATPEPCK, anticorps Ppc1C, anticorps PHOSPHOENOLPYRUVATE CARBOXYKINASE, anticorps T28I19.150, anticorps T28I19_150, anticorps phosphoenolpyruvate carboxykinase 1, anticorps 143299_at, anticorps CG10924, anticorps CG17725, anticorps Dmel\\CG17725, anticorps Dromel_CG17725_FBtr0086701_pepck_mORF, anticorps dPEPCK, anticorps pepck, anticorps PEPC, anticorps PEPCase, anticorps ppc, anticorps phosphoenolpyruvate carboxykinase 1, anticorps phosphoenolpyruvate carboxykinase 2, mitochondrial, anticorps phosphoenolpyruvate carboxykinase 2 (mitochondrial), anticorps phosphoenolpyruvate carboxykinase 1 (soluble), anticorps phosphoenolpyruvate carboxykinase 1 S homeolog, anticorps phosphoenolpyruvate carboxykinase, cytosolic [GTP], anticorps phosphoenolpyruvate carboxykinase 1, cytosolic, anticorps phosphoenolpyruvate carboxylase 7, anticorps Phosphoenolpyruvate carboxykinase, anticorps phosphoenolpyruvate carboxylase, anticorps PCK1, anticorps PCK2, anticorps Pck2, anticorps pck1, anticorps pck1.S, anticorps LOC100634531, anticorps Pck1, anticorps pep7, anticorps Pepck, anticorps LOC107777405
- Sujet
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Synonyms: PEPC 1 antibody, PEPCase 1 antibody, Phosphoenolpyruvate carboxylase 1 antibody
Background: Phospho Enol Pyruvate Carboxylase is an enzyme in the family of carboxy-lyases that catalyzes the addition of bicarbonate to phosphoenolpyruvate (PEP) to form a four-carbon compound oxaloacetate. This reaction is used for carbon fixation in CAM and C4 plants where it plays a key role in photosynthesis. The enzyme is also found in some bacteria, but not in animals or fungi.
Gene Name: PEPC
- ID gène
- 542372
- UniProt
- B8XPZ2
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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